Pentadecanoic acid against Candida albicans-Klebsiella pneumoniae biofilm: towards the development of an anti-biofilm coating to prevent polymicrobial infections

• DOI: 10.1016/j.resmic.2021.103880
• Publication Date: 2021-09-24T09:18:26Z
• Authors: E. Galdiero; A. Ricciardelli; C. D'Angelo; E. de Alteriis; A. Maione; L. Albarano; A. Casillo; M.M. Corsaro; M.L. Tutino; E. Parrilli
• Keywords: Pentadecanoic acid

The ability to form biofilms is a common feature of microorganisms, which can colonize variety surfaces, such as host tissues and medical devices, resulting in infections highly resistant conventional drugs. This aspect particularly critical polymicrobial involving both fungi bacteria, therefore, eradicate severe infections, new effective anti-biofilm strategies are needed. efficacy pentadecanal pentadecanoic acid agents has been recently reported against different bacterial strains. Their chemical similarity with diffusible signal factors (DSFs), plus the already known fatty acids act agents, suggested explore their use Candida albicans Klebsiella pneumoniae mixed biofilm. In this work, we demonstrated molecules prevent formation destabilize structure dual-species Moreover, coating, previously developed through adsorption on polydimethylsiloxane (PDMS), was proved biofilm dynamic conditions by confocal laser scanning microscopy analysis. Finally, evaluation expression levels some biofilm-related genes C. K. treated provided insights into molecular mechanisms underpinning its effect.