Xenogeneic mesenchymal stem cell biocurative improves skin wounds healing in diabetic mice by increasing mast cells and the regenerative profile

• DOI: 10.1016/j.reth.2022.12.006
• Publication Date: 2023-01-09T23:16:31Z
• Authors: Gabriel Martins d...; Jefferson Elias-O...; Jhefferson Barbos...; Ítalo Sousa Pereira; Vanessa Fernandes...; Beatriz Burger; Daianne Maciely C...; Lucas Eduardo Bot...; Hosana Gomes Rodr...; Vânia Luiza Deper...; João Santana Silva; Simone Gusmão Ramos; Rita Cassia Tostes; Adriana Oliveira ...; Carolina Caliari-...; Daniela Carlos
• Keywords: Proinflammatory cytokine; Cell therapy

Diabetes mellitus (DM) is a chronic disease and major cause of mortality morbidity worldwide. The hyperglycemia caused by DM induces micro macrovascular complications that lead, among other consequences, to wounds amputations. Cell therapy tissue engineering constitute recent therapeutic alternatives improve wound healing in diabetic patients. current study aimed analyze the effectiveness biocuratives containing human mesenchymal stem cells (MSCs) associated with hydrogel matrix process related inflammatory cell profile mice.Biocuratives MSCs were constructed 3D bioprinting, applied skin on back streptozotocin (STZ)-induced type 1 (T1D) mice. process, after application or without was histologically analyzed. In parallel, genes growth factors, mast (MC), M1 M2 macrophage profiles evaluated RT-PCR. Macrophages characterized flow cytometry, MC toluidine blue staining cytometry.Mice T1D exhibited fewer delayed when compared non-diabetic group. Treatment accelerated improved collagen deposition Increased TGF-β gene expression macrophage-related markers also detected mice received MSCs-containing biocuratives. Finally, upregulated IL-33 augmented number mice.These results reveal potential mice, providing scientific base for future treatments