Surface modulation of extracellular vesicles with cell-penetrating peptide-conjugated lipids for improvement of intracellular delivery to endothelial cells

• DOI: 10.1016/j.reth.2022.12.007
• Publication Date: 2023-01-11T07:04:51Z
• Authors: Tianwei Huang; Yuya Sato; Akiko Kuramochi; Yoshio Ohba; Masayuki Sano; Makoto Miyagishi; Hiroaki Tateno; Renu Wadhwa; Kazunori Kawasaki; Takeyuki Uchida; Kristina N. Ekdahl; Bo Nilsson; Ung-il Chung; Yuji Teramura
• Keywords: Exosome; Fluorescein isothiocyanate

Exosomes (diameter 30-200 nm) are a subtype of extracellular vesicles secreted by cells containing DNA, microRNA (miRNA), and proteins. expected to be valuable as means delivering drugs or functional miRNAs in treatment diseases. However, the delivery exosomes is not sufficiently effective, even though have intrinsic functions. Cell-penetrating peptides (CPPs) short peptide families that facilitate cellular intake molecules vesicles. We previously reported modification cells, liposomes with CPP-conjugated-lipids, CPPs conjugated poly (ethylene glycol)-conjugated phospholipids (PEG-lipid), induce adhesion CPPs, can useful for cell-based assays harvesting liposomes. In this study, we aimed modulate exosome surface using Tat (YGRKKRRQRRR)-PEG-lipids improve intracellular endothelial cells. isolated characterized from medium HEK 293 T cell cultures. PEG-lipids different spacer molecular weights lipid types were incorporated into fluorescein isothiocyanate labeling optimize number Tat-PEG-lipids immobilized on surface. The modified incubated human umbilical vein (HUVECs) study interaction. 5 kDa PEG C16 lipids highly detected inside HUVECs flow cytometry. Fluorescence was negligible control groups. Thus, surface, improving exosomes.