Comparative evaluation of the effects of different activating media and temperatures on European eel (Anguilla anguilla) sperm motility assessed by computer assisted sperm analysis

• DOI: 10.1016/j.rvsc.2023.105045
• Publication Date: 2023-10-05T00:51:50Z
• Authors: Alberto Elmi; Antonio Casalini; Martina Bertocchi; Pietro Emmanuele; Camilla Aniballi; Albamaria Parmegg...; Nadia Govoni; Domenico Ventrella; Oliviero Mordenti; Maria Laura Bacci
• Keywords: Artificial fertilization

The European eel is a critically endangered teleost fish with very poor success rate for captive breeding and artificial reproduction. Therefore, to support its conservation, new strategies are needed ensure fertilization. Objective analysis of sperm motility may be critical as it potentially represents one the most important reproductive quality parameters. Spermatozoa acquire once in contact hyperosmotic solutions saltwater, yet exact mechanisms role temperature still clarified. main aim study was assess effects 3 activating media (artificial sea water, tank water commercial Actifish®) at 4 20 °C on motility, by means computer assisted analysis. Secondary test 2 different concentrations Actifish® mimicking pH/osmolality, °C. results suggested how both have spermatozoa kinematics, mainly acting upon interaction type. samples activated showed poorest outcomes (mean 38.1%), while ones diluted 1:4 °C, highest (means 51.8 51.5% respectively). Additionally, diluting reach same pH osmolality seawater led worse outcomes, suggesting that composition factor activation rather than itself.