The effects of abiotic stressors and signal molecules on phenolic composition and antioxidant activities of in vitro regenerated Hypericum perforatum (St. John's Wort)

• DOI: 10.1016/j.sajb.2020.07.037
• Publication Date: 2020-08-24T15:55:15Z
• Authors: Nese Eray; Abdullah Dalar; Musa Turker
• Keywords: Chlorogenic Acid; Hypericum; Hyperforin

Hypericum perforatum L. is a pharmaceutically and industrially significant natural source due to its rich phenolic content antioxidant potential. This study aimed establish an optimized development protocol through plant tissue culture methods evaluate the effects of abiotic stressors signal molecules on composition responses. Methanol based extracts prepared from lyophilized powders samples obtained nature aseptic seedlings, calluses stress factors were analysed for using HPLC-MS/MS responses complementary (FCR, FRAP ORAC). Experimental analysis revealed use B5 SH media in vitro regeneration as most proper chemical environments standardization perforatum. Chromatographic findings showed that quercetin, pseudohypericin partially chlorogenic acid compounds key The analyses deduced UV sucrose increased level acid, while ascorbic quercetin drought applications concentration compared control. Rutin compound was only produced pot samples, which indicates response stress. Additionally, it found B5, SH, MS containing 2 mg/L NAA+1 mg BA with elicitors 50 µmol 6% 5 (or 10) min-24 h harvesting might be optimization Findings within this novel contributions optimization, production antioxidative pharmaceutical industrial utilization.