Antibacterial mechanism of Eugenia stipitata McVaugh essential oil and synergistic effect against Staphylococcus aureus

• DOI: 10.1016/j.sajb.2022.03.012
• Publication Date: 2022-03-12T17:16:37Z
• Authors: Wêndeo Kennedy Costa; Alisson Macário d...; Izabelly Bianca d...; Valquíria Bruna G...; Elys Karine Carva...; João Victor de Ol...; Ana Paula Sant'An...; Vera Lúcia de Men...; Maria Tereza dos ...; Márcia Vanusa da ...
• Keywords: Efflux; Membrane permeability

The purpose of this study was to evaluate the antibacterial and antivirulence activities an essential oil from E. stipitata (EsEO) leaves against Staphylococcus aureus as well its mechanism action. determination activity based on MIC MBC. explored with growth curve, synergistic effect, cell membrane permeability assays. In addition, antimicrobial in a model S. aureus-infected Tenebrio mollitor larvae cytotoxicity fibroblasts macrophages were evaluated. MBC EsEO 128–512 μg/mL 256–1024 μg/mL, respectively. curve test showed that inhibited by EsEO. effect all investigated antibiotics high capacity reduce hemolysis (78.18-92.48%) staphyloxanthin (67.27-91.89%). increase efflux ions, proteins, nucleic acids revealed integrity had been affected. promoted protection T. infected aureus. did not present cytotoxic effects machophages fibroblast human cells demonstrated strains including multidrug resistant strains.