S-metolachlor promotes oxidative stress in green microalga Parachlorella kessleri - A potential environmental and health risk for higher organisms

• DOI: 10.1016/j.scitotenv.2018.04.433
• Publication Date: 2018-05-09T02:05:51Z
• Authors: Dubravka Špoljari...; Ivna Štolfa Čamag...; Janja Horvatić; Tanja Žuna Pfeiffer; Filip Stević; Neven Žarković; Georg Waeg; Morana Jaganjac
• Keywords: 0301 basic medicine; Aldehydes; Parachlorella kessleri ; S-metolachlor ; Reactive oxygen species ; 4-Hydroxy-2-nonenal ; Lipid peroxidation ; Antioxidants; 6. Clean water; 3. Good health; Oxidative Stress; 03 medical and health sciences; Chlorophyta; 13. Climate action; Acetamides; Microalgae; Lipid Peroxidation; Water Pollutants, Chemical

The estimation of the toxic influences of herbicide products on non-target aquatic organisms is essential for evaluation of environmental contamination. We assessed the effects of the herbicide S-metolachlor (S-MET) on unicellular green microalga Parachlorella kessleri during 4-72 in vitro exposure to concentrations in the range 2-200μg/L. The results have shown that S-MET had a significant effect on algae, even in doses 10 and 20 times lower than the EC50 values obtained for P. kessleri (EC50-72h=1090μg/L). It generates reactive oxygen species in algae, decreases their growth and photosynthetic pigment concentration, changes their ultrastructure and alters the cellular antioxidant defence capacities. The levels of protein adducts with the reactive aldehyde 4-hydroxy-2-nonenal (HNE), the end-product of lipid peroxidation, were significantly elevated in S-MET treated cells revealing the insufficient effectiveness of P. kessleri antioxidant mechanisms and persistent lipid peroxidation. Since algae are fundamental aquatic food component, the damaged algal cells, still capable of dividing while having persistently increased content of HNE upon S-MET contamination could represent an important environmental toxic factor that might further affect higher organisms in the food chain.