Metabolic engineering of Yarrowia lipolytica for poly(ethylene terephthalate) degradation

• DOI: 10.1016/j.scitotenv.2022.154841
• Publication Date: 2022-03-29T00:33:24Z
• Authors: Katarzyna E. Kosi...; Piotr Biniarz; Adam Dobrowolski; Karol Leluk; Aleksandra M. Mir...
• Keywords: Cutinase; Yarrowia; Degradation; Strain (injury)

Polyethylene terephthalate (PET) is the most widely used plastic, whose global production scale causes serious problems due to it being highly non-biodegradable. The present work provides a novel approach plastic degradation studies, which involves direct of PET in culture modified Y. lipolytica yeast strain extracellularly producing cutinase from Fusarium solani. In this study, we successfully accomplished scale-up process culture, promising perspective wider application developed method future. Additionally, tested effect various supplements, may increase efficiency pAD CUT_FS strain. ability decomposition was verified by amount released products, such as terephthalic acid (TPA) and mono-(2-hydroxyethyl)-terephthalic (MHET), during cultivation. We observed that quantities TPA MHET were increasing daily, 1.51 gL-1 0.45 gL-1, respectively after 240 h bioreactor fermentation. Analysis film electron microscopy indicated there abundant damage on surface material. This study also demonstrated engineered able degrade at 28 °C results obtained using amorphous powder provide wide range possibilities for cutinase-secreting more difficult crystalline films, bottles melts.