Generation of three miR-122 knockout lines from a human embryonic stem cell line

• DOI: 10.1016/j.scr.2017.06.004
• Publication Date: 2017-06-10T10:15:19Z
• Authors: Yanli Liu; Feima Wu; Yuanqi Zhuang; Dongsheng Guo; Nasir Abbas; Ruzi Wei; Kepin Wang; Yan Chen; Jiawang Tao; Yuhang Wu; Fang Yuan; Tingcai Pan; Fan Yang; Keyu Lai; Liangxue Lai; Yin-xiong Li
• Keywords: 0301 basic medicine; MicroRNAs; 03 medical and health sciences; QH301-705.5; Human Embryonic Stem Cells; Humans; Biology (General); Cell Line

miR-122 is the most abundant miRNA in the human liver, accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 plays key roles in hepatocyte growth, metabolism, and homeostasis. Here, we created three miR-122 knockout human embryonic stem cell line lines, WAe001-A-7, WAe001-A-8, and WAe001-A-9, using the CRISPR/Cas9 technique. These mutated cell lines retained their pluripotency, in vitro differentiation potential, normal morphology, and karyotype.