Pluripotent pig embryonic stem cell lines originating from in vitro-fertilized and parthenogenetic embryos

• DOI: 10.1016/j.scr.2020.102093
• Publication Date: 2020-11-20T03:41:54Z
• Authors: Kwang-Hwan Choi; Dong-Kyung Lee; Jong-Nam Oh; Seung-Hun Kim; Mingyun Lee; Sang-Ho Woo; Dae-Yong Kim; Chang-Kyu Lee
• Keywords: 0301 basic medicine; Mice; 03 medical and health sciences; QH301-705.5; Swine; Parthenogenesis; Animals; Mice, Nude; Cell Differentiation; Biology (General); Embryonic Stem Cells; Cell Line

Here, we report in vivo developmental competent pig embryonic stem cells (ESCs) originating from in vitro-fertilized and parthenogenetic embryos cultured in chemically defined culture media. The pig ESC lines expressed pluripotency markers and were capable of forming teratomas following subcutaneous injection into nude mice. These cell lines would be a useful resource for comparative developmental biology and agricultural biotechnology.