In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells

• DOI: 10.1016/j.scr.2021.102233
• Publication Date: 2021-02-11T02:29:07Z
• Authors: Masayoshi Yoshimatsu; Hiroe Ohnishi; Chengzhu Zhao; Yasuyuki Hayashi; Fumihiko Kuwata; Shinji Kaba; Hideaki Okuyama; Yoshitaka Kawai; Nao Hiwatashi; Yo Kishimoto; Tatsunori Sakamoto; Makoto Ikeya; Koichi Omori
• Keywords: Chondrogenesis; Thyroid cartilage

The laryngotracheal cartilage is a cardinal framework for the maintenance of airway breathing, which occasionally requires reconstruction. Because hyaline has poor intrinsic regenerative ability, various approaches have been attempted to regenerate cartilage. use autologous mesenchymal stem cells (MSCs) regeneration widely investigated. However, long-term culture may limit proliferative capacity. Human-induced pluripotent cell-derived MSCs (iMSCs) can circumvent this problem due their unlimited This study aimed investigate efficacy iMSCs in thyroid immunodeficient rats. Herein, we induced through neural crest cell intermediates. For relevance prospective future clinical application, induction was conducted under xeno-free/serum-free conditions. Then, clumps fabricated from an iMSC/extracellular matrix complex (C-iMSC) were transplanted into defects Histological examinations revealed cartilage-like regenerated tissue and human nuclear antigen (HNA)-positive surviving lesion. HNA-positive co-expressed SOX9, type II collagen identified around cells. These results indicated that C-iMSCs promoted some differentiated chondrogenic lineage Induced be promising candidate therapy