Establishment and characterization of human induced pluripotent stem cell line (WMUi020-A) from a patient with bicuspid aortic valve aortopathy

• DOI: 10.1016/j.scr.2021.102260
• Publication Date: 2021-02-21T15:47:25Z
• Authors: Li Dong; Huan Zhang; Yingyi Quan; Peifeng Jin; Jiwen Yang; Xiaofang Fan; Junming Fan; Ming Li; Yongsheng Gong; Yongyu Wang
• Keywords: Reprogramming; Germ layer

A human induced pluripotent stem cell (hiPSC) line (WMUi020-A) was generated from the aortic smooth muscle cells of a 56-year-old donor with bicuspid valve and ascending aneurysm. Episomal vector-mediated Non-integration iPSC reprogramming used for this generation. The established highly expressed pluripotency markers three germ-layer differentiation potential in vitro, as well normal karyotype. We further found that has mutation ROBO4 (c.161 T>C, p.Q54R), which may be useful disease modeling aortopathy.