Establishment of an induced pluripotent stem cell line (JTUi003-A) from a patient with otosclerosis

• DOI: 10.1016/j.scr.2022.102666
• Publication Date: 2022-01-15T15:58:16Z
• Authors: Yuanping Xiong; Ying Wang; Maoxiang Xu; Yaoqian Liu; Hongqun Jiang; Zhuang Jiang; Jiping Wang
• Keywords: Male; 0301 basic medicine; QH301-705.5; Induced Pluripotent Stem Cells; Cell Differentiation; Middle Aged; Cell Line; 03 medical and health sciences; Otosclerosis; Leukocytes, Mononuclear; Humans; Biology (General); Germ Layers

Otosclerosis is characterized by abnormal bone remodeling in the osseous labyrinth and progressive hearing loss. Although the etiology of otosclerosis is not fully understood, both environmental and genetic factors play important roles in its pathogenesis. Here, we generated an induced pluripotent stem cell line using episomal plasmid vectors from the peripheral blood mononuclear cells of a 48-year-old male with otosclerosis. The morphology and karyotype of the cells were normal. The expression of pluripotency markers was verified by mRNA and protein levels; the pluripotency state of the cell line was verified by successful differentiation into all three germ layers.