Generation of ultra-treatment-resistant schizophrenia patient-derived induced pluripotent stem cell line UJSi002-A

• DOI: 10.1016/j.scr.2022.102766
• Publication Date: 2022-03-29T00:55:05Z
• Authors: Jing Sun; Rongsen Zeng; Yunxi Liu; Siyu Xi; Tingkai Sun; Jinjun Qian; Zhenghui Yi; Shengying Qin
• Keywords: Germ layer; Homeobox protein NANOG

Schizophrenia is a chronic, serious and disabling mental disorder. Most patients can effectively control their condition through drug treatment, but there are still some who difficult to gain benefits from treatment. Among them, the failure respond clozapine full-scale treatment ultra-treatment-resistant schizophrenia. We generated induced pluripotent stem cells (iPSCs) an schizophrenia patient by electroporation of peripheral blood mononuclear (PBMCs) with episomal plasmids encoding OCT 4, SOX 2, NANOG, LIN 28, KLF 4 MYC. The iPSCs demonstrated normal karyotype, expressed pluripotency markers differentiated into three germ layers in vivo.