Generation of heterozygous (MCRIi031-A-1) and homozygous (MCRIi031-A-2) SOX9 knockout human iPSC lines

• DOI: 10.1016/j.scr.2024.103484
• Publication Date: 2024-06-22T22:37:00Z
• Authors: Svenja Pachernegg; Gorjana Robevska; Lucas G. A. Ferreira; Jocelyn A. van de...; Katerina Vlahos; Sara E. Howden; Andrew H. Sinclair; Katie L. Ayers
• Keywords: Reprogramming; Germ layer; SOX9; Gene knockout

The transcription factor SOX9 plays a critical role in several embryonic developmental processes such as gonadogenesis, chrondrogenesis, and cardiac development. We generated heterozygous (MCRIi031-A-1) homozygous (MCRIi031-A-2) knockout induced pluripotent stem cell (iPSC) lines from human fibroblasts using one-step protocol for CRISPR/Cas9 gene-editing episomal-based reprogramming. Both iPSC exhibit normal karyotype morphology, express pluripotency markers, have the capacity to differentiate into three germ layers. These will allow us further explore of processes.