Generation of a homozygous (MCRIi031-A-3) WT1 knockout human iPSC line

• DOI: 10.1016/j.scr.2024.103494
• Publication Date: 2024-07-11T00:06:51Z
• Authors: Svenja Pachernegg; Gorjana Robevska; Lucas G.A. Ferreira; Jocelyn A. van de...; Katerina Vlahos; Sara E. Howden; Andrew H. Sinclair; Katie L. Ayers
• Keywords: Reprogramming; Germ layer; Gene knockout

The transcription factor WT1 plays a critical role in several embryonic developmental processes such as gonadogenesis, nephrogenesis, and cardiac development. We generated homozygous (MCRIi031-A-3) knockout induced pluripotent stem cell (iPSC) line from human fibroblasts using one-step protocol for CRISPR/Cas9 gene-editing episomal-based reprogramming. cells exhibit normal karyotype morphology, express pluripotency markers, have the capacity to differentiate into three germ layers. These lines will allow us further explore of processes.