Bioaerosol Exposure and in vitro Activation of Toll-like Receptors in a Norwegian Waste Sorting Plant

• DOI: 10.1016/j.shaw.2021.09.002
• Publication Date: 2021-09-29T21:45:41Z
• Authors: Elke Eriksen; Pål Graff; Ine Pedersen; Anne Straumfors; Anani K. Afanou
• Keywords: Bioaerosol; Indoor bioaerosol; Limulus amebocyte lysate

The global shift toward greener societies demands new technologies and work operations in the waste-management sector. However, progressive industrial methods do not necessarily consider workers' health. This study characterized exposure to bioaerosols investigated bioaerosols' potential engage immune system vitro.Full personal aerosol sampling was conducted over three consecutive days. Dust load analyzed by gravimetry, fungal actinobacterial spores were scanning electron microscopy, endotoxin limulus amebocyte lysate (LAL) assay. In vitro of HEK cells airborne dust samples used investigate inducing an inflammatory reaction.The total level exceeded recommended occupational limit (OEL) 5.0 mg/m3 3 out 15 samples. inhalable a 7-fold, whereas spore 11-fold. Actinobacterial identified 8 14 experiments revealed significant TLR2 activation 9 vs. TLR4 all samples.The present showed that contained potentially health-impairing endotoxin, fungi, levels. Furthermore, sampled microbial components capable TLR thus have evoke response exposed individuals.