Detection of SARS-CoV-2 by using real-time PCR nasopharyngeal swabs in suspected patients and their clinical medication

• DOI: 10.1016/j.sintl.2021.100148
• Publication Date: 2021-12-06T17:56:45Z
• Authors: Muhammad Waqar Ma...; Ahmad Raza; Tayyaba shaheen; Muhammad Zubair; Javaria Mahmood; Hira Tahir; Saira Saif; Fatima Mazhar
• Keywords: LDH (Lactate dehydrogenase); TG (Triglyceride); SARS-CoV-2; 0202 electrical engineering, electronic engineering, information engineering; HDL (High density lipid); Electrical engineering. Electronics. Nuclear engineering; 02 engineering and technology; Covid-19; Article; CRP (c reactive protein); TK1-9971; 3. Good health

The corona name derived from their crown like spike proteins attach with cell receptors. It belongs to coronaviradae family and nideovirales order, envelop virus, size range 65-125 ​nm positive single standard RNA between 26.4 31.7 ​kb contain 7096 amino acid. There are four subtypes that have been detected these alpha, beta, gamma delta.The 267 covid-19 blood nasopharyngeal samples were collected Multan region. extraction run the PCR. use for clinical tests, Lactate dehydrogenase, serum ferritin level, D-Dimer, TG, cholesterol, thyphoidot, HDL, lymphocyte count CRP.127 (47.21%) out of patients PCR showed amplification ORF1ab, E, N gene, while 140 individuals negative not E gene. Covid-19 PCR, other analysis tests such as lactate ferritin, ESR, CBP, Tg, CRP CT scan. effected higher values Neutrophils, LDH, level than normal ranges. However, cholesterol lymphocytes decreased range.