All-electrical antibiotic susceptibility testing within 30 min using silicon nano transistors

• DOI: 10.1016/j.snb.2022.131458
• Publication Date: 2022-01-21T16:10:52Z
• Authors: Xingxing Xu; Si Chen; Yingtao Yu; Petra Virtanen; Jiyue Wu; Qitao Hu; Sanna Koskiniemi; Zhen Zhang
• Keywords: 0301 basic medicine; Minimal inhibitory concentration; Cell metabolism; 03 medical and health sciences; Analytisk kemi; Antibiotic susceptibility testing; Point of care; Ion-selective field-effect transistor; Analytical Chemistry

Rapid and reliable antibiotic susceptibility testing (AST) platform is highly desired to select the right antibiotics to treat infectious disease at early stage. Here, we demonstrate rapid ASTs using nanoscale silicon ion-selective field-effect transistor sensors. Our sensors profile bacterial metabolic kinetics by monitoring the metabolism induced acidification in the growth media with the absence and the presence of different antibiotics. Rapid AST results could be determined from the metabolic profiles with a total assay time less than 30 min for different bacterial strains. In addition, the sensors could also distinguish the bactericidal mechanisms for antibiotics with different modes of actions. Furthermore, the initial bacterial concentration in an unknown sample, a key parameter to determine its clinic relevance, could be estimated based on the metabolic profiles. Our demonstrated AST method is all-electrical, label-free and silicon technology compatible, and holds great promise for the development of a high-throughput and low-cost point-of-care device.