Spatiotemporal single-cell roadmap of human skin wound healing

• DOI: 10.1016/j.stem.2024.11.013
• Publication Date: 2024-12-26T15:39:53Z
• Authors: Zhuang Liu; Xiaowei Bian; Lihua Luo; Åsa K. Björklund; Li Li; Letian Zhang; Yongjian Chen; Lei Guo; Juan Gao; Chunyan Cao; Jiating Wang; Wenjun He; Yunting Xiao; Liping Zhu; Karl Annusver; Nusayhah Hudaa Gopee; Daniela Basurto-L...; David Horsfall; Clare L. Bennett; Maria Kasper; Muzlifah Haniffa; Pehr Sommar; Dongqing Li; Ning Xu Landén

Wound healing is vital for human health, yet the details of cellular dynamics and coordination in wound repair remain largely unexplored. To address this, we conducted single-cell multi-omics analyses on skin tissues through inflammation, proliferation, remodeling phases from same individuals, monitoring molecular at an unprecedented spatiotemporal resolution. This singular roadmap reveals architecture margin identifies FOSL1 as a critical driver re-epithelialization. It shows that pro-inflammatory macrophages fibroblasts sequentially support keratinocyte migration like relay race across different stages. Comparison with data venous diabetic foot ulcers uncovers link between failed impaired inflammatory response chronic wounds. Additionally, comparing mouse acute transcriptomes underscores indispensable value this bridging basic research clinical innovations.