Activation of GLP-1 Receptor Promotes Bone Marrow Stromal Cell Osteogenic Differentiation through β-Catenin

• DOI: 10.1016/j.stemcr.2016.02.002
• Publication Date: 2016-03-03T19:36:17Z
• Authors: Jingru Meng; Xue Ma; Ning Wang; Min Jia; Long Bi; Yunying Wang; Mingkai Li; Huinan Zhang; Xiaoyan Xue; Zheng Hou; Ying Zhou; Zhibin Yu; Gonghao He; Xiaoxing Luo
• Keywords: Male; Medicine (General); QH301-705.5; Immunoblotting; Active Transport, Cell Nucleus; Article; Bone and Bones; Glucagon-Like Peptide-1 Receptor; Rats, Sprague-Dawley; 03 medical and health sciences; R5-920; Bone Density; Osteogenesis; exendin-4; Animals; Biology (General); Cell Nucleus; 0303 health sciences; Microscopy, Confocal; Osteoblasts; Reverse Transcriptase Polymerase Chain Reaction; Correction; Cell Differentiation; Mesenchymal Stem Cells; osteoporosis; osteoblast; Exenatide; Osteoporosis; RNA Interference; Peptides; glucagon-like peptide 1 receptor; bone marrow stromal cells; Signal Transduction

Glucagon-like peptide 1 (GLP-1) plays an important role in regulating bone remodeling, and GLP-1 receptor agonist shows a positive relationship with osteoblast activity. However, is not found osteoblast, the mechanism of on remodeling unclear. Here, we show that exendin-4 (Ex-4) promoted formation increased mass quality rat unloading-induced loss model. These functions were accompanied by increase number serum markers, while adipocyte was decreased. Furthermore, detected marrow stromal cells (BMSCs), but osteoblast. Activation Ex-4 osteogenic differentiation inhibited BMSC adipogenic through PKA/β-catenin PKA/PI3K/AKT/GSK3β signaling. findings reveal regulates provide molecular basis for therapeutic potential against osteoporosis.