Transcriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells

• DOI: 10.1016/j.stemcr.2017.12.005
• Publication Date: 2018-01-04T16:47:33Z
• Authors: Chee Jia Chin; Suwen Li; Mirko Corselli; David Casero; Yuhua Zhu; Chong Bin He; Reef Hardy; Bruno Péault; Gay M. Crooks
• Keywords: CD146; Mesenchyme; CD90

Various mesenchymal cell types have been identified as critical components of the hematopoietic stem/progenitor (HSPC) niche. Although several groups described generation mesenchyme from human pluripotent stem cells (hPSCs), capacity such to support hematopoiesis has not reported. Here, we demonstrate that distinct subpopulations co-emerge mesoderm during hPSC differentiation. Despite co-expression common markers (CD73, CD105, CD90, and PDGFRβ), a subset defined CD146hiCD73hi expressed genes associated with HSPC niche supported maintenance functional HSPCs ex vivo, while CD146loCD73lo Stromal was contact dependent mediated in part through high JAG1 expression low WNT signaling. Molecular profiling revealed significant transcriptional similarity between hPSC-derived CD146++ primary perivascular cells. The derivation functionally diverse hPSCs opens potential avenues model develop PSC-based therapies.