SOX10 Single Transcription Factor-Based Fast and Efficient Generation of Oligodendrocytes from Human Pluripotent Stem Cells

• DOI: 10.1016/j.stemcr.2017.12.014
• Publication Date: 2018-01-11T16:42:02Z
• Authors: Juan Antonio Garc...; Manoj Kumar; Ruben Boon; David Chau; Jennifer One; Esther Wolfs; Kristel Eggermont; Pieter Berckmans; Nilhan Gunhanlar; Femke de Vrij; Bas Lendemeijer; Benjamin Pavie; Nikky Corthout; Steven A. Kushner; José Carlos Dávila; Ivo Lambrichts; Wei-Shou Hu; Catherine M. Verf...
• Keywords: SOX10

Scarce access to primary samples and lack of efficient protocols generate oligodendrocytes (OLs) from human pluripotent stem cells (hPSCs) are hampering our understanding OL biology the development novel therapies. Here, we demonstrate that overexpression transcription factor SOX10 is sufficient surface antigen O4-positive (O4+) myelin basic protein-positive OLs hPSCs in only 22 days, including patients with multiple sclerosis or amyotrophic lateral sclerosis. The SOX10-induced O4+ population resembles at transcriptome level can myelinate neurons vivo. Using vitro OL-neuron co-cultures, myelination by also be demonstrated, which adapted a high-throughput screening format test response pro-myelinating drugs. In conclusion, provide an approach very rapid manner, used for disease modeling, drug discovery efforts, potentially therapeutic transplantation.