miR-34b/c Regulates Wnt1 and Enhances Mesencephalic Dopaminergic Neuron Differentiation

• DOI: 10.1016/j.stemcr.2018.02.006
• Publication Date: 2018-03-08T16:45:55Z
• Authors: Roberto De Gregorio; Salvatore Pulcrano; Claudia De Sanctis; Floriana Volpicelli; Ezia Guatteo; Lars von Oerthel; Emanuele Claudio ...; Roberta Esposito; Rosa Maria Piscit...; Carla Perrone-Capano; Valerio Costa; Dario Greco; Stefano Puglisi-A...; Marten P. Smidt; Umberto di Porzio; Massimiliano Caiazzo; Nicola Biagio Mer...; Meng Li; Gian Carlo Bellenchi
• Keywords: Tetrodotoxin

The differentiation of dopaminergic neurons requires concerted action morphogens and transcription factors acting in a precise well-defined time window. Very little is known about the potential role microRNA these events. By performing microRNA-mRNA paired microarray screening, we identified miR-34b/c among most upregulated microRNAs during differentiation. Interestingly, modulates Wnt1 expression, promotes cell cycle exit, induces When combined with ASCL1 NURR1, doubled yield transdifferentiated fibroblasts into neurons. Induced (iDA) cells synthesize dopamine show spontaneous electrical activity, reversibly blocked by tetrodotoxin, consistent electrophysiological properties featured brain Our findings point to for neuronal commitment highlight exploiting its synergy key enhancing vitro generation