Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells

• DOI: 10.1016/j.stemcr.2018.03.012
• Publication Date: 2018-04-12T15:45:57Z
• Authors: Oleh V. Halaidych; Christian Freund; Francijna van den...; Daniela C.F. Salv...; Mara Riminucci; Christine L. Mummery; Valeria V. Orlova
• Keywords: Regenerative Medicine

Several studies have reported endothelial cell (EC) derivation from human induced pluripotent stem cells (hiPSCs). However, few explored their functional properties in depth with respect to line-to-line and batch-to-batch variability how they relate primary ECs. We therefore carried out accurate characterization of hiPSC-derived ECs (hiPSC-ECs) multiple (non-integrating) hiPSC lines compared them various assays, which included barrier function using real-time impedance spectroscopy an integrated assay electric wound healing, endothelia-leukocyte interaction under physiological flow mimic inflammation angiogenic responses vitro vivo assays. Overall, we found many similarities but also some important differences between Assessment vasculogenic showed little difference hiPSC-ECs regard blood vessel formation, may be future regenerative medicine applications requiring vascularization.