MSX2 Initiates and Accelerates Mesenchymal Stem/Stromal Cell Specification of hPSCs by Regulating TWIST1 and PRAME

• DOI: 10.1016/j.stemcr.2018.06.019
• Publication Date: 2018-07-20T09:39:40Z
• Authors: Leisheng Zhang; Hongtao Wang; Cuicui Liu; Qingqing Wu; Pei Su; Dan Wu; Jiaojiao Guo; Wen Zhou; Yuanfu Xu; Lihong Shi; Jiaxi Zhou
• Keywords: Regenerative Medicine

The gap in knowledge of the molecular mechanisms underlying differentiation human pluripotent stem cells (hPSCs) into mesenchymal cell lineages hinders application hPSCs for cell-based therapy. In this study, we identified a critical role muscle segment homeobox 2 (MSX2) initiating and accelerating program that leads to stem/stromal (MSC) from hPSCs. Genetic deletion MSX2 impairs hPSC MSCs. When aided with cocktail soluble molecules, ectopic expression induces form nearly homogeneous fully functional Mechanistically, neural crest cells, an intermediate stage preceding MSCs, further by regulating TWIST1 PRAME. Furthermore, found is also required MSCs through mesendoderm trophoblast. Our findings provide novel mechanistic insights lineage specification effective strategies applications regenerative medicine.