GATA2 Promotes Hematopoietic Development and Represses Cardiac Differentiation of Human Mesoderm

• DOI: 10.1016/j.stemcr.2019.07.009
• Publication Date: 2019-08-08T14:34:12Z
• Authors: Julio Castaño; Sergi Aranda; Clara Bueno; Fernando J. Caler...; Eva Mejia-Ramirez; Jose Luis Mosquera; Enrique Blanco; Xiaonan Wang; Cristina Prieto; Lorea Zabaleta; Elisabetta Mereu; Meritxell Rovira; Senda Jiménez-Del...; Daniel R. Matson; Holger Heyn; Emery H. Bresnick; Berthold Göttgens; Luciano Di Croce; Pablo Menendez; Angel Raya; Alessandra Giorgetti
• Keywords: GATA2

In vertebrates, GATA2 is a master regulator of hematopoiesis and expressed throughout embryo development in adult life. Although the essential role mouse well established, its involvement during early human hematopoietic not clear. By combining time-controlled overexpression with genetic knockout experiments, we found that GATA2, at mesoderm specification stage, promotes generation hemogenic endothelial progenitors their further differentiation to progenitor cells, negatively regulates cardiac differentiation. Surprisingly, genome-wide transcriptional chromatin immunoprecipitation analysis showed bound regulatory regions, repressed expression development-related genes. Moreover, genes important for were upregulated by mostly indirect manner. Collectively, our data reveal hitherto unrecognized as repressor fates, highlight importance coordinating repression alternative cell fates.