Aberrant nucleosome organization in mouse SCNT embryos revealed by ULI-MNase-seq

• DOI: 10.1016/j.stemcr.2022.05.020
• Publication Date: 2022-06-23T14:43:13Z
• Authors: Lingyue Yang; Xiaocui Xu; Ruimin Xu; Chuan Chen; Xiaolei Zhang; Mo Chen; Xiaochen Kou; Yanhong Zhao; Hong Wang; Xiaoyu Liu; Shaorong Gao; Chong Li
• Keywords: Totipotent; Reprogramming

Somatic cell nuclear transfer (SCNT) can reprogram terminally differentiated somatic cells into totipotent embryos, but with multiple defects. The nucleosome positioning, as an important epigenetic regulator for gene expression, is largely unexplored during SCNT embryonic development. Here, we mapped genome-wide profiles in mouse embryos using ultra-low-input MNase-seq (ULI-MNase-seq). We found that the nucleosome-depleted regions (NDRs) around promoters underwent dramatic reestablishment, which consistent cycle. Dynamics of position were delayed compared to fertilized embryos. Subsequently, aberrant expression levels inner mass (ICM) positively correlated promoter NDRs donor cells, indicated memory occupancy was a potential barrier SCNT-mediated reprogramming. further confirmed histone acetylation level associated NDRs. Our study provides insight reconfiguration preimplantation