Oligodendrocytes in human induced pluripotent stem cell-derived cortical grafts remyelinate adult rat and human cortical neurons

• DOI: 10.1016/j.stemcr.2023.04.010
• Publication Date: 2023-05-25T14:54:32Z
• Authors: Raquel Martinez-C...; Linda Jansson; Oleg Tsupykov; Natalia Avaliani; Constanza Aretio-...; Isabel Hidalgo; Emanuela Monni; Johan Bengzon; Galyna Skibo; Olle Lindvall; Zaal Kokaia; Sara Palma-Tortosa
• Keywords: Neuroepithelial cell; Human brain; Neural cell

Neuronal loss and axonal demyelination underlie long-term functional impairments in patients affected by brain disorders such as ischemic stroke. Stem cell-based approaches reconstructing remyelinating neural circuitry, leading to recovery, are highly warranted. Here, we demonstrate the vitro vivo production of myelinating oligodendrocytes from a human induced pluripotent stem cell (iPSC)-derived neuroepithelial (lt-NES) line, which also gives rise neurons with capacity integrate into stroke-injured, adult rat cortical networks. Most importantly, generated survive form myelin-ensheathing axons host tissue after grafting onto organotypic cultures. This lt-NES line is first source that, intracerebral delivery, can repair both injured circuitries demyelinated axons. Our findings provide supportive evidence for potential future use iPSC-derived lines promote effective clinical recovery following injuries.