Minichromosome maintenance protein 10 (mcm10) regulates hematopoietic stem cell emergence in the zebrafish embryo

• DOI: 10.1016/j.stemcr.2023.05.022
• Publication Date: 2023-07-11T14:47:34Z
• Authors: Pietro Cacialli; Serkan Dogan; Tanja Linnerz; Corentin Pasche; Julien Y. Bertrand
• Keywords: Minichromosome maintenance; Minichromosome

Hematopoietic stem cells (HSCs) guarantee the continuous supply of all blood lineages during life. In response to stress, HSCs are capable extensive proliferative expansion, whereas in steady state, largely remain a quiescent state prevent their exhaustion. DNA replication is very complex process, where many factors need exert functions perfectly concerted manner. Mini-chromosome-maintenance protein 10 (Mcm10) an important factor, required for proper assembly eukaryotic fork. this report, we use zebrafish study role mcm10 embryonic development, and show that specifically regulates HSC emergence from hemogenic endothelium. We demonstrate mcm10-deficient embryos present accumulation damages nascent HSCs, inducing apoptosis. This phenotype can be rescued by knocking down p53. Taken together, our results plays definitive hematopoiesis.