O-GlcNAcase regulates pluripotency states of human embryonic stem cells

• DOI: 10.1016/j.stemcr.2024.05.009
• Publication Date: 2024-06-27T14:31:28Z
• Authors: Qianyu Liu; Cheng Chen; Zhiya Fan; Honghai Song; Yutong Sha; Liyang Yu; Yingjie Wang; Weijie Qin; Wen Yi
• Keywords: Pluripotent Stem Cells; Glycosylation; Human Embryonic Stem Cells; Humans; Cell Differentiation; Article; beta-N-Acetylhexosaminidases; Acetylglucosamine; Cell Line

Understanding the regulation of human embryonic stem cells (hESCs) pluripotency is critical to advance field developmental biology and regenerative medicine. Despite recent progress, molecular events regulating hESC pluripotency, especially transition between naive primed states, still remain unclear. Here we show that hESCs display lower levels O-linked N-acetylglucosamine (O-GlcNAcylation) than hESCs. O-GlcNAcase (OGA), key enzyme catalyzing removal O-GlcNAc from proteins, highly expressed in important for pluripotency. Depletion OGA accelerates naive-to-primed transition. transcriptionally regulated by EP300 acts as a transcription regulator genes maintaining Moreover, profile protein O-GlcNAcylation two states quantitative proteomics. Together, this study identifies an factor suggests has broad effect on homeostasis.