Antagonistic effect of epigallocatechin-3-gallate on neurotoxicity induced by formaldehyde

• DOI: 10.1016/j.tox.2018.10.022
• Publication Date: 2018-11-03T01:45:50Z
• Authors: Jiawei Huang; Yu Lu; Bin Zhang; Shaoping Yang; Qian Zhang; Haiyan Cui; Xianxian Lu; Yun Zhao; Xu Yang; Rui Li
• Keywords: Male; 0303 health sciences; Behavior, Animal; NF-E2-Related Factor 2; Brain; Membrane Proteins; Catechin; 3. Good health; Mice; 03 medical and health sciences; Neuroprotective Agents; Formaldehyde; NAD(P)H Dehydrogenase (Quinone); Animals; Cognitive Dysfunction; Neurotoxicity Syndromes; Maze Learning; Heme Oxygenase-1

The toxicity of formaldehyde (FA) has always been of great concern, particularly since its use is unavoidable. On the other hand, epigallocatechin-3-gallate (EGCG), an active substance in tea polyphenols, has been shown to demonstrate physiological protective functions by in both epidemiological and zoological studies, particularly in the nervous system. The study described here, aims to explore whether EGCG can alleviate the neurotoxic effects induced by formaldehyde. After 14 days of exposure to 3 mg/m3 formaldehyde, mice exhibited significant cognitive impairment. In the FA group, a significant increase in iNOS level compared with the control group was observed. The reduced GSH level was significantly decreased. The levels of IL-1β, TNF-α and Caspase-3 were obviously raised, while H&E and Nissl staining illustrated significant neuronal damage. After administering EGCG as a protective agent, all the above observed changes were reversed, and the protective effect of EGCG became gradually evident in the 20-500 mg/kg range. Immunohistochemistry results showed that EGCG could activate the Nrf2 signaling pathway, thus alleviating the oxidative damage caused by formaldehyde.