Lead exposure-induced cognitive impairment through RyR-modulating intracellular calcium signaling in aged rats

• DOI: 10.1016/j.tox.2019.03.005
• Publication Date: 2019-03-21T14:03:55Z
• Authors: Lu Ouyang; Wei Zhang; Guihua Du; Haizhen Liu; Jie Xie; Junwang Gu; Shuyun Zhang; Fankun Zhou; Lijian Shao; Chang Feng; Guangqin Fan
• Keywords: Male; Neurons; 0301 basic medicine; Behavior, Animal; Age Factors; Apoptosis; Hippocampus; 3. Good health; Rats, Sprague-Dawley; Disease Models, Animal; 03 medical and health sciences; Cognition; Lead Poisoning, Nervous System, Adult; Escape Reaction; Organometallic Compounds; Animals; Cognitive Dysfunction; Female; Calcium Signaling; Phosphorylation; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Cyclic AMP Response Element-Binding Protein; Maze Learning

Lead is widely distributed in the environment and has become a global public health issue. It is well known that lead exposure induces not only neurodevelopmental toxicity but also neurodegenerative diseases, with learning and memory impairment in the later stage. However, the molecular mechanisms remain elusive. The present study investigated the effects of early life and lifetime lead exposure on cognition and identified the molecular mechanisms involved in aged rats. The results herein demonstrated that the lead concentration in peripheral blood and brain tissues in aged rats was significantly increased in a lead dose-dependent manner. High-dose lead exposure caused cognitive functional impairment in aged rats, concomitant with a longer escape latency and a lower frequency of crossing the platform via Morris water maze testing compared to those in the control and low-dose lead exposure groups. Importantly, neuron functional defects were still observed even in early life lead exposure during the prenatal and weaning periods in aged rats. The neurotoxicity induced by lead exposure was morphologically evidenced by a recessed nuclear membrane, a swollen endoplasmic reticulum, and mitochondria in the neurons. Mechanistically, the exposure of aged rats to lead resulted in increasing free calcium concentration, reactive oxygen species, and apoptosis in the hippocampal neurons. Lead exposure increased RyR3 expression and decreased the levels of p-CaMKIIα/CaMKIIα and p-CREB/CREB in the hippocampus of aged rats. These findings indicated that early life lead exposure-induced cognition disorder was irreversible in aged rats. Lead-induced neurotoxicity might be related to the upregulation of RyR3 expression and high levels of intracellular free calcium with increasing lead concentration in injured neurons.