The development of an in vitro 3D model of goblet cell hyperplasia using MUC5AC expression and repeated whole aerosol exposures

• DOI: 10.1016/j.toxlet.2021.04.012
• Publication Date: 2021-04-21T10:13:49Z
• Authors: Linsey E. Haswell; David Smart; Tomasz Jaunky; Andrew Baxter; Simone Santopietro; Stuart Meredith; Oscar M. Camacho; Damien Breheny; David Thorne; Marianna D. Gaca
• Keywords: Goblet cell

Goblet cell hyperplasia and overproduction of airway mucin are characteristic features the lung epithelium smokers COPD patients. Tobacco heating products (THPs) a potentially less risky alternative to combustible cigarettes, through continued use solus THPs may reduce smoking-related disease risk. Using MucilAir™ in vitro model, 6-week feasibility study was conducted investigating effect repeated cigarette smoke (1R6F), THP aerosol air exposure. Tissues were exposed nicotine-matched whole doses 3 times/week. Endpoints assessed dosimetry, tight-junction integrity, cilia beat frequency (CBF) active area (AA), cytokine secretion MUC5AC expression. Comparison incubator controls indicated exposures did not have significant on transepithelial electrical resistance (TEER), CBF AA tissues. Cytokine clear differences patterns response 1R6F exposure resulted decrease TEER (p=0.000 p=0.000, respectively), an increase positive cells (p=0.002). Repeated result change cells. This demonstrates can induce these morphological changes vitro.