Imidacloprid exposure cause the histopathological changes, activation of TNF-α, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpio L.)

• DOI: 10.1016/j.toxrep.2017.12.019
• Publication Date: 2017-12-27T08:23:47Z
• Authors: Selçuk Özdemir; Serdar Altun; Harun Arslan
• Keywords: Common carp; Neonicotinoid

Imidacloprid (IMI) is a neonicotinoid that widely used for the protection of crops and carnivores from insects parasites, respectively. It well known imidacloprid exposure has harmful effect on several organisms. However, there little information about toxicity in aquatic animals, particularly fish. Thus, current study, we assessed histopathological changes; activation iNOS, 8-OHdG TNF-α; expression levels caspase 3, CYP1A MT1 genes common carp exposed to imidacloprid. For this purpose, fish were either low dose (140 mg/L) or high (280 24 h, 48 72 h 96 h. After IMI exposure, detected hyperplasia secondary lamellar cells mucous cell gills, as hydropic degeneration hepatocytes necrosis liver. Moreover, 8-OHdG, iNOS TNF-α was found gills liver but also moderately brain. Transcriptional analysis showed 3 altered doses (p < 0.05), up-regulated with both time-dependent manner 0.05, p 0.01, 0.001), not significantly changed regardless time > 0.05) except lastly, gene only brain 0.01). Our results indicated acute induce apoptosis caused severe lesions, inflammation, oxidative stress liver, carp.