Effects of USP7 on radiation sensitivity through p53 pathway in laryngeal squamous cell carcinoma

• DOI: 10.1016/j.tranon.2022.101466
• Publication Date: 2022-06-10T20:45:24Z
• Authors: Hao Niu; Yi Zhu; Jie Wang; Tian Wang; Xiaosheng Wang; Li Yan
• Keywords: Radioresistance; Radiosensitivity; Radiation sensitivity; Wild type

The relationship between ubiquitin specific protease 7 (USP7) and radio-sensitivity in laryngeal squamous cell carcinoma (LSCC) has not been reported yet. Using gene chip Label-Free mass spectrometry, we found that USP7 was significantly increased both radioresistant LSCC patients cells receiving irradiation. Since p53 is the most important downstream of frequently mutated LSCC, investigated effects on radioresistance with or without mutation. We knockdown p53-mutated cells, while inhibiting p53-wild type cells. Knockdown inhibited expression pathway. Overexpressing endogenous by CRISPR/dCas9 could reverse radiosensitivity vitro vivo. Our results demonstrated irradiation-induced led to but Therefore, clinical application inhibitors may improve radiotherapy mutations reduce side surrounding normal tissues