Association of blood donor characteristics with in vitro platelet storage properties

• DOI: 10.1016/j.transci.2023.103746
• Publication Date: 2023-06-01T20:05:10Z
• Authors: Ravneet Kaur; Kshitija Mittal; Rajbir Kaur Cheema; Seema Gupta; Tanvi Sood; Paramjit Kaur
• Keywords: Male; Blood Platelets; Adult; Young Adult; Glucose; Adolescent; Blood Preservation; Humans; Blood Donors; Lactic Acid; Middle Aged; Aged

The study was planned to determine the association of blood donor characteristics with in vitro quality of platelets.In the prospective observational study, a total of 85 male whole blood donors in the age group of 18-30 and 45-65 years were enrolled using purposive sampling method. Serum total cholesterol, glycosylated hemoglobin (HbA1c), and LDH levels were performed on donor pre-donation sample. Buffy coat platelet concentrates were prepared from 450 mL quadruple blood bags. Samples from platelets were taken on day one and five of storage and biochemical properties were observed.Median MPV was higher in platelets from older blood donors on day five (9.8 vs 9.4, p = 0.037). Median LDH levels were also higher in platelets on day one and five from older donors (Day one: 204.5 vs 147, p = <0.000; day five: 278 vs 224, p = 0.001 respectively). Platelets from donors with high HbA1c levels had lower median pH (Day one: 7.31 vs 7.37, p = 0.024) and higher median glucose levels on day one of storage (Day one: 358 vs 311, p = 0.001). Higher median lactate levels throughout the storage period were also seen in platelets from donors with higher HbA1c levels (Day one: 7 vs 5.7, p = 0.037; Day five: 16 vs 12.2, p = 0.032). Glucose consumption (108 vs 66, p = 0.025) and lactate production (9 vs 6.4, p = 0.019) was higher in platelets from donors with higher HbA1c levels.In vitro platelet storage properties are affected by blood donor characteristics.