Pre- and Posttransplant Monitoring of Alloantibodies by Complement-Dependent Cytotoxicity and Luminex Methodologies in Liver Transplantation

• DOI: 10.1016/j.transproceed.2012.07.005
• Publication Date: 2012-09-29T07:49:57Z
• Authors: F. Monteiro; H. Rodrigues; J. Kalil; M.C. Castro; N. Panajotopoulos; M. Paredes; P. Massarolo; S. Mies
• Keywords: Cytotoxicity, Immunologic; Graft Rejection; Transplantation; B-Lymphocytes; Time Factors; Histocompatibility Testing; T-Lymphocytes; Cytotoxicity Tests, Immunologic; Fluorescence; Liver Transplantation; 3. Good health; 03 medical and health sciences; Treatment Outcome; 0302 clinical medicine; HLA Antigens; Isoantibodies; Monitoring, Immunologic; Predictive Value of Tests; Histocompatibility; Humans; Surgery; Complement Activation; Biomarkers

This study evaluated the influence of circulating anti-HLA antibodies on outcomes of 97 liver allografts from deceased donors.Human leukocyte antigen (HLA) antibody screening was performed by both complement-dependent cytotoxicity (CDC) and multiparameter Luminex microsphere-based assays (Luminex assay).The agreements between T- and B- cell CDC and Luminex assays were 67% and 77% for pre- and posttransplant specimens, respectively. Graft dysfunction was not associated with either positive pretransplant CDC or Luminex panel-reactive antibody (PRA) values. Likewise, positive posttransplant T- or B- cell CDC PRA values were not associated with graft dysfunction. In contrast, posttransplant Luminex PRA values were significantly higher among patients with graft dysfunction compared with subjects with good outcomes (P = .017).Posttransplant monitoring of HLA antibodies with Luminex methodology allowed identification of patients at high-risk for poor graft outcomes.