Cytokines and chemokines in cerebrospinal fluid in relation to diagnosis, clinical presentation and recovery in children being evaluated for Lyme neuroborreliosis

• DOI: 10.1016/j.ttbdis.2020.101390
• Publication Date: 2020-01-27T16:36:07Z
• Authors: Barbro H Skogman; Malin Lager; Lars Brudin; Maria C Jenmalm; Ivar Tjernberg; Anna J Henningsson
• Keywords: Lyme Neuroborreliosis; Neuroborreliosis; Presentation (obstetrics); LYME

In Lyme neuroborrelios (LNB), the immune response has been in focus, but association between different cytokines/chemokines and clinical manifestations LNB patients not fully investigated. The aim of this study was to evaluate a large number cytokines chemokines cerebrospinal fluid (CSF) relation diagnosis, presentation recovery children being evaluated for LNB. Pediatric (n = 105) were recruited at seven Swedish pediatric departments during 2010-14. Serum CSF samples drawn on admission, before start antibiotic treatment. Patients diagnosed as Definite or Possible categorized LNBtot patients, all presented with pleocytosis CSF. Non-LNB Other diagnosis Controlstot, controlstot without Multiplex bead array (Luminex) kits used analyses 41 (Millipore). Twenty-eight detectable levels 26 these mediators significantly higher than Controlstot. discriminant analysis, combination four (CXCL1, GM-CSF, IL-7 IL-10) shown independently separate relevant patient groups. Furthermore, an IL-10/CXCL1 ratio created have improved diagnostic performance distinguishing vs compared CXCL13 No mediator differed significantly, when comparing admission within 2 months admission. A analysis be useful distinguish most important CSF, order discriminate from patients. An promising profile better chemokine However, further evaluation is required address future possible usefulness laboratory diagnostics LNB, including control groups neuro-inflammation. significant associations found