Ultrasound-assisted depolymerization of kappa-carrageenan and characterization of degradation product

• DOI: 10.1016/j.ultsonch.2021.105540
• Publication Date: 2021-03-26T23:45:28Z
• Authors: Mariel G. Tecson; Lucille V. Abad; Virgilio D. Ebajo; Drexel H. Camacho
• Keywords: Depolymerization; Carrageenan; Degradation; Gel permeation chromatography

Degradation of polysaccharides to afford low-molecular-weight oligosaccharides have been shown produce new bioactivities that are not present in the starting material. The simplicity ultrasonic treatment degradation a polysaccharide, such as κ-carrageenan, offers practical advantage producing degraded products with lower molecular weight may interesting potential activities. This study embarked on investigating effects weights and structural changes κ-carrageenan under varying conditions. Molecular (MW) monitoring ultrasonically-treated at various conditions were done by gel permeation chromatography. product formed using optimized condition was characterized FTIR NMR. decrease MW has be dependent low concentration (5.0 mg mL−1), high amplitude (85%), long time (180 mins) average (AMW) 41,864 Da, which is 96.33% reduction from raw sample initial AMW 1,139,927 Da. Structural analysis reveals most peaks retained minor change. 1D 2D NMR analyses showed sonic process afforded where sulfate group G4S-4 position cleaved forming methylene G4S ring. results would useful structure–activity relationship understanding effect applications κ-carrageenan.