Ultrasonic-assisted extraction of polyphenolic compounds from Paederia scandens (Lour.) Merr. Using deep eutectic solvent: optimization, identification, and comparison with traditional methods

• DOI: 10.1016/j.ultsonch.2022.106005
• Publication Date: 2022-04-12T01:28:42Z
• Authors: Yuxin Liu; Wang Zhe; Ruifen Zhang; Ziting Peng; Yuxi Wang; Heqi Gao; Zhiqiang Guo; Juan Xiao
• Keywords: ABTS; Deep eutectic solvent; Choline chloride

Ultrasonic-assisted extraction (UAE) coupled with deep eutectic solvent (DES) is a novel, efficient and green method for phytochemicals. In this study, the effects of 16 DESs UAE on rate polyphenols from Paederia scandens (Lour.) Merr. (P. scandens), an edible medicinal herb, were investigated. DES synthesised choline chloride ethylene glycol at 1:2 M ratio resulted in highest extractability. Moreover, parameters investigated by using two-level factorial experiment followed response surface methodology The optimal (water content 49.2%, actual ultrasonic power 72.4 W, time 9.7 min) total flavonoid (TFC) (27.04 mg CE/g DW), ferric-reducing antioxidant (FRAP) value (373.27 μmol Fe(Ⅱ)E/g DW) 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid radical (ABTS+) (48.64 TE/g closely matching experimental results. Furthermore, comparison study demonstrated that DES-UAE afforded higher TFC FRAP than traditional methods. 36 individual polyphenolic compounds identified quantified ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) P. extracts, which 30 found extracts obtained DES-UAE. Additionally, sum compound content. These results revealed enhanced efficiency provided scientific basis further processing utilization scandens.