Poliovirus replicons encoding the B subunit of Helicobacter pylori urease protect mice against H. pylori infection

• DOI: 10.1016/j.vaccine.2004.07.037
• Publication Date: 2004-09-16T14:10:39Z
• Authors: Lesley E. Smythies; Miroslav J. Novak; Ken B. Waites; J. Russell Lindsey; Casey D. Morrow; Phillip D. Smith
• Keywords: 0301 basic medicine; Helicobacter pylori; Urease; Helicobacter Infections; 3. Good health; Mice, Inbred C57BL; Mice; Poliovirus; 03 medical and health sciences; Bacterial Vaccines; Vaccines, Subunit; Animals; Humans; Replicon; HeLa Cells

We developed a novel vaccine for Helicobacter pylori based on a poliovirus vector in which capsid genes were replaced with the gene for the B subunit of H. pylori urease (UreB). Mice were vaccinated with UreB or control (L1) replicon and challenged with H. pylori. Twenty percent of mice vaccinated prophylactically with UreB, but 80% vaccinated with L1, and then challenged with H. pylori became infected (P = 0.003). Seventy-three percent of mice with established H. pylori infection vaccinated therapeutically with UreB replicon cleared their infection compared to 33% vaccinated with L1 (P = 0.067). In therapeutically vaccinated mice with residual infection, UreB-vaccinated animals had fewer H. pylori than L1-vaccinated mice (P < 0.05). Anti-urease antibody titres in prophylactically, but not therapeutically, vaccinated mice were markedly higher in animals that received UreB versus L1 replicon (P = 0.01). Vaccination with poliovirus vector containing the gene for the B subunit of H. pylori urease provides significant prophylactic and strong therapeutic protection against H. pylori in mice.