Multivalent liposome-based vaccines containing different serosubtypes of PorA protein induce cross-protective bactericidal immune responses against Neisseria meningitidis

• DOI: 10.1016/j.vaccine.2005.07.056
• Publication Date: 2005-08-04T03:13:47Z
• Authors: Holly E. Humphries; Jeannette N. Will...; Ramona Blackstone; Keith A. Jolley; Ho M. Yuen; Myron Christodoul...; John E. Heckels
• Keywords: Mice, Inbred BALB C; Vaccines, Synthetic; 0303 health sciences; Immune Sera; Porins; Meningococcal Vaccines; Cross Reactions; Neisseria meningitidis; Antibodies, Bacterial; 3. Good health; Mice; 03 medical and health sciences; 616; Liposomes; Animals; Serotyping

Four serosubtypes (P1.7, 16, P1.7-2, 4, P1.19, 15 and P1.5-1, 10-4) of the PorA outer-membrane protein of Neisseria meningitidis were purified as recombinant proteins and incorporated into liposomes to investigate their immunogenicity. Each serosubtype induced high levels of bactericidal activity against the homologous strain. In addition, liposome preparations containing multiple serosubtypes induced high levels of bactericidal activity against each of the four strains. Significantly, antisera raised against monovalent and multivalent liposomes also showed cross-reactive bactericidal activity against heterologous strains. These data demonstrate that multivalent liposome vaccines, containing multiple PorA serosubtypes, have the potential to provide protection against a broad range of meningococcal strains.