Effect of oral administration of ginseng stem-and-leaf saponins (GSLS) on the immune responses to Newcastle disease vaccine in chickens

• DOI: 10.1016/j.vaccine.2011.04.097
• Publication Date: 2011-05-12T15:12:01Z
• Authors: Lijuan Zhai; Yutao Li; Weiyu Wang; Yuemin Wang; Songhua Hu
• Keywords: Male; 0301 basic medicine; 2. Zero hunger; Newcastle Disease; Newcastle disease virus; Administration, Oral; Panax; Viral Vaccines; Hemagglutination Inhibition Tests; Saponins; Antibodies, Viral; Vaccines, Attenuated; Immunoglobulin A; 3. Good health; 03 medical and health sciences; Adjuvants, Immunologic; Animals, Newborn; Animals; Lymphocytes; Intestinal Mucosa; Chickens; Poultry Diseases; Cell Proliferation

This study was designed to evaluate the effect of oral administration of ginseng stem-and-leaf saponin (GSLS) on immune responses in chickens vaccinated with live Newcastle disease (ND) vaccines. In experiment 1, chickens were orally administered GSLS (2.5, 5, 10, and 20mg/kg) in drinking water for 3 days, and then intranasally vaccinated with ND vaccine. Hemagglutination inhibition (HI) test showed that the optimal dose of GSLS for enhancing HI titers was 5mg/kg. In experiment 2, chickens were fed with GSLS (5mg/kg) for 7, 5 or 3 days before vaccination. Result indicated that GSLS enhanced HI titers in a time-dependent manner. The order of the duration for drinking GSLS on increasing HI titers was 7 days>5 days>3 days. In experiment 3, chickens were fed with GSLS before or after immunization. Serum was sampled at a week interval for 5 weeks for HI test, splenic lymphocytes were prepared at weeks 2 and 4 after the booster immunization for lymphocyte proliferation test (LPT) and intestinal tissues were sampled at week 4 after the booster immunization for immunohistochemistry and histological examination. Results showed that HI titer, LPT, IgA+ cells and intestinal intraepithelial lymphocytes (iIELs) were significantly higher in chickens drinking GSLS before vaccination than those after vaccination. Therefore, oral administration of GSLS in drinking water at a dose of 5mg/kg for 7 days significantly increased systemic as well as gut mucosal immunity in chickens immunized with ND vaccine. Considering the immune potentiating effect of GSLS on ND vaccine, GSLS should be evaluated for its oral adjuvant effect on the immune response against other diseases such avian influenza and infectious bursal disease.