Omp16-based vaccine encapsulated by alginate-chitosan microspheres provides significant protection against Haemophilus parasuis in mice

• DOI: 10.1016/j.vaccine.2017.01.067
• Publication Date: 2017-02-08T01:46:43Z
• Authors: Xintian Zheng; Xiaoyan Yang; Xiaohua Li; Guo-Hua Qiu; Ailing Dai; Qichun Huang; Cuiqin Huang; Xiaofeng Guo
• Keywords: 0301 basic medicine; Antigens, Bacterial; Chitosan; Drug Carriers; Immunity, Cellular; Mice, Inbred BALB C; Haemophilus Infections; Alginates; Hexuronic Acids; Animal Structures; Antibodies, Bacterial; Bacterial Load; Immunity, Humoral; 3. Good health; Disease Models, Animal; Haemophilus parasuis; 03 medical and health sciences; Glucuronic Acid; Leukocytes, Mononuclear; Animals; Cytokines; Bacterial Outer Membrane Proteins; Haemophilus Vaccines

Haemophilus parasuis (H. parasuis) is the etiological agent of swine Glässer's disease, which leads to significant economic loss in swine industry over the world. Subunit vaccine based on outer membrane protein is one of the promising choices to protect pigs against H. parasuis infection despite low immunity efficiency. In this paper, outer membrane protein 16 (Omp16) of H. parasuis encapsulated by alginate-chitosan microspheres as antigen carriers was explored for the first time in a mouse model. Our results showed that the microspheres with Omp16 induced significant higher H. parasuis-specific antibodies, and higher titers of IL-2, IL-4, and IFN-γ than those by Omp16-FIA in treated mice (p<0.05). Moreover, H. parasuis load in the tissues from liver, spleen, and lung of mice immunized with microspheres containing Omp16 was significantly decreased (p<0.05) than that in the same counterpart tissues of control groups. In addition, 80% mice treated with Omp16 and 70% mice with Omp16-FIA were survived after challenged with H. parasuis virulent strain LY02 (serovar 5). Therefore, Omp16-based microsphere vaccine induces both humoral and cellular immune responses and provides promising protection against H. parasuis infection in mice.