Phylogenetic identification of feline leukemia virus A and B in cats with progressive infection developing into lymphoma and leukemia

• DOI: 10.1016/j.virusres.2023.199093
• Publication Date: 2023-03-20T05:34:29Z
• Authors: Giovana Biezus; Thierry Grima de ...; Gabriela Bassi da...; Mariana da Silva ...; Paula Barros Brizola; Marina Silvestre ...; Luiz Claudio Miletti; Renata Assis Casa...
• Keywords: Feline leukemia virus

To date, only a few studies have examined the impacts of feline leukemia virus (FeLV) subgroups on disease development in spontaneously infected cats. The present study identified FeLV-A and FeLV-B cats with lymphoma explored phylogenetic relationships env sequences. Twenty-six (n=16) or (n=10) were selected. FeLV p27 antigen positivity was determined using ELISA, proviral DNA blood samples detected nested PCR. Positive animals both tests classified as cases progressive infection subjected to second PCR for amplification subgroup determination. Six five sequenced Sanger method, results used build tree estimate evolutionary divergence. Among lymphoma, 68.8% carried FeLV-AB 31.2% FeLV-A. leukemia, 70% 30% Regarding cat characteristics, 50% young, 30.8% young adults, 19.2% adults; 88.5% mixed-breed 11.5% pure breed; 42.3% males 57.7% females. lymphomas, 62.5% mediastinal, 31.3% multicentric, 6.3% extranodal. histological classification, lymphoblastic small non-cleaved-cell lymphomas most frequently detected. cases, acute lymphoid, chronic myeloid, 40% myeloid. Phylogenetic analysis showed that SC sequences closely related Arena, Glasgow-1, FeLV-FAIDS variants. Meanwhile, divergent from one another but similar endogenous FELV gene (enFeLV). In conclusion, is prevalent highlighting genetic diversity involved pathogenesis these neoplasms Brazil.