Inhibition of EV71 replication by an interferon-stimulated gene product L3HYPDH

• DOI: 10.1016/j.virusres.2024.199336
• Publication Date: 2024-02-13T23:58:20Z
• Authors: Jian Liu; Logen Liu; Shinuan Zeng; Xiaobin Meng; Nanfeng Lei; Hai Yang; Runcai Li; Xin Mu; Xuemin Guo
• Keywords: L3HYPDH; 0303 health sciences; 03 medical and health sciences; Interferon-stimulated gene; Enterovirus 71; Infectious and parasitic diseases; RC109-216; Antiviral activity; Microbiology; QR1-502; Article; 3. Good health

AbstractEnterovirus 71 (EV71) is the common causative agent of hand-foot-mouth disease (HFMD). Despite evidence in mice model suggested that the interferon (IFN) signaling pathways play a role in defending against this virus, knowledge on the IFN-mediated antiviral response is still limited. Here we identified an IFN-stimulated gene (ISG) called L3HYPHD, whose expression inhibits EV71 replication. Mapping assay indicated that amino acids 61-120 and 295-354 are critical for its optimal antiviral activity. Mechanismly, L3HYPDH specifically inhibits protein translation mediated by EV71 internal ribosome entry site (IRES). Our data thus uncovered a new mechanism utilized by the host cell to restrict EV71 replication.