A single-cell atlas of the healthy breast tissues reveals clinically relevant clusters of breast epithelial cells

• DOI: 10.1016/j.xcrm.2021.100219
• Publication Date: 2021-03-19T01:45:13Z
• Authors: Poornima Bhat-Nak...; Hongyu Gao; Liu Sheng; Patrick C. McGuire; Xiaoling Xuei; Jun Wan; Yunlong Liu; Sandra K. Althouse; Austyn Colter; George Sandusky; Anna Maria Storniolo; Harikrishna Naksh...
• Keywords: Reduction Mammoplasty; Breast tissue

Single-cell RNA sequencing (scRNA-seq) is an evolving technology used to elucidate the cellular architecture of adult organs. Previous scRNA-seq on breast tissue utilized reduction mammoplasty samples, which are often histologically abnormal. We report a rapid collection/processing protocol perform biopsies healthy women and identify 23 epithelial cell clusters. Putative cell-of-origin signatures derived from these clusters applied analyze transcriptomes ~3,000 cancers. Gene mature luminal enriched in ~68% cancers, whereas signature progenitor cluster ~20% Overexpression cluster-derived HER2+, but not other subtypes, associated with unfavorable outcome. TBX3 PDK4 as genes co-expressed estrogen receptor (ER) normal breasts, their expression analyses >550 cancers enable prognostically relevant subclassification ER+