Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease

• DOI: 10.1016/j.xcrm.2024.101556
• Publication Date: 2024-05-21T15:12:55Z
• Authors: Suvi Linna-Kuosmanen; Eloi Schmauch; Kyriakitsa Galani; Johannes Ojanen; Carles A. Boix; Tiit Örd; Anu Toropainen; Prosanta K. Singha; Pierre R. Moreau; Kristiina Harju; Adriana Blazeski; Åsa Segerstolpe; Veikko Lahtinen; Lei Hou; Kai Kang; Elamaran Meibalan; Leandro Z. Agudelo; Hannu Kokki; Jari Halonen; Juho Jalkanen; Jarmo Gunn; Calum A. MacRae; Maija Hollmén; Juha E.K. Hartika...; Minna U. Kaikkonen; Guillermo García-...; Pasi Tavi; Tuomas Kiviniemi; Manolis Kellis
• Keywords: Proinflammatory cytokine; Ex vivo

Cardiovascular disease plays a central role in the electrical and structural remodeling of right atrium, predisposing to arrhythmias, heart failure, sudden death. Here, we dissect with single-nuclei RNA sequencing (snRNA-seq) spatial transcriptomics gene expression changes human ex vivo atrial tissue pericardial fluid ischemic disease, myocardial infarction, non-ischemic failure using asymptomatic patients valvular who undergo preventive surgery as control group. We reveal substantial differences disease-associated all cell types, collectively suggesting inflammatory microvascular dysfunction composition vascular diseases progress into failure. The data suggest that investigation cardiovascular should expand functionally important parts heart, which may help us identify mechanisms promoting more severe types disease.