Reaching the potential of electron diffraction

• DOI: 10.1016/j.xcrp.2024.102007
• Publication Date: 2024-05-23T14:34:00Z
• Authors: Devrim Acehan; Katherine A. Spoth; Gabrielle R. Budz...; M. Elizabeth Snell; Christopher S. Ca...; Miranda L. Lynch; Sarah E.J. Bowman
• Keywords: Cryo-Electron Microscopy

Microcrystal electron diffraction (MicroED) is an emerging structural technique in which submicron crystals are used to generate data for studies. Structures allow the study of molecular-level architecture and drive hypotheses about modes action, mechanisms, dynamics, interactions with other molecules. Combining cryoelectron microscopy (cryo-EM) instrumentation crystallographic techniques, MicroED has led three-dimensional models small molecules, peptides, proteins generated tremendous interest due its ability use vanishingly crystals. In this perspective, we describe current state field methodologies, including making detecting appropriate size technique, as well ways best handle characterize these Our perspective provides insight into unlock full range potential access previously intractable samples describes areas future development.